CULTURE OF TWO-CELL RABBIT EMBRYOS TO MORULAE

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Author:
R. L. BRINSTER
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DOI:
https://doi.org/10.1530/jrf.0.0210017
Volume/Issue:
Volume 21: Issue 1
Page Range:
17–22
Article Type:
Research Article
Online Publication Date:
Feb 1970
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Summary.

The rabbit embryo is able to develop from the two-cell stage to the morula in a medium containing an amino-nitrogen source as the only metabolizable component. This amino-nitrogen source can be bovine serum albumin, oxidized glutathione, or certain single amino acids such as glutamine, proline or alanine. Although a requirement for a non-nitrogenous energy source could not be demonstrated during this period, a beneficial effect of pyruvate and lactate was shown. The optimum concentration for pyruvate was approximately 10 −4 m and for lactate was 1 to 3×10−2m.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899