The antigenicity of male bat reproductive organs was studied using agar-gel diffusion. Four precipitin lines formed with testis and anti-testis, six lines with epididymis and anti-epididymis, and six lines with ampulla and anti-ampulla sera. Tissue extracts of liver, spleen, heart and uterus cross-reacted with the three antisera to produce three lines. Ampulla, epididymis and testis possessed at least one, three and one organ-specific antigens, respectively.
Nine proteins were detected in the seminal vesicles by agar-gel electrophoresis. One of these possessed potent toxic properties when injected into rabbits and mice. The toxic protein was isolated by zonal electrophoresis and by chromatography on Sephadex G-200. It migrated electrophoretically as a pre-albumin, had a molecular weight of approximately 44,600 and an LD50 value of 162μg/kg. The relationship between this protein and the unique phenomenon of bat sperm survival is unknown.
If the inline PDF is not rendering correctly, you can download the PDF file here.