Fructose was determined in ejaculates of normal and vasectomized male mice, after recovery of the ejaculates from mated females. Approximately 70% of the total fructose remained in the copulatory plug and only 30% was present in the fluid portion of the seminal plasma. When the fluid portion of the ejaculate was left in the uterus for 2 hr without contact with the plug (the uterine horn having been ligated), a statistically significant decrease of fructose occurred in relation to the initial value. When, however, such semen was left in the uterus in contact with the plug, this decrease was counterbalanced by an influx of fructose released from the plug.
No clear difference in the decrease of fructose content was found between the fluid portion of ejaculates from normal and vasectomized males after 2 hr in the uterus.
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