Trophoblast grafts were disrupted 5, 8 and 10 days after the transplantation of 3½-day-old fertilized mouse ova beneath the kidney capsule of syngeneic mouse recipients. Cell suspensions or teased preparations of these grafts were stained with the Feulgen technique for deoxyribonucleic acid (DNA). The results of cytophotometric measurements of Feulgen-DNA in individual cell nuclei showed that between 5 and 10 days after grafting, the trophoblast nuclei exhibited massive increases in Feulgen-DNA. Although possible stoichiometric artifacts could not be completely excluded, the results indicated that trophoblast giant-cell formation does involve large increases in nuclear DNA.
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