The suggestion has been made in recent years that dihydrotestosterone (DHT : 17 β-hydroxy-5α-androstan-3-one) may be the biologically active form of testosterone in a variety of target organs. The chick comb, for example, achieves an appreciable conversion of testosterone to DHT (Gloyna & Wilson, 1969). With this in mind, several experiments were performed to compare the actions of testosterone propionate (TP) and DHT in the fowl, using comb weight, testis weight, anterior pituitary weight and gonadotrophin content as end-points for androgen activity.
Single comb, White Leghorn fowl were used in the experiments and the steroids (TP and DHT) were suspended in sesame oil. At autopsy, the glands were quickly removed and weighed. The anterior pituitary glands were stored in acetone for total gonadotrophin assay or were homogenized in cold distilled