Electrical stimulation of the anterior hypothalamus of the vole, Microtus agrestis, usually results in ovulation, whereas stimulation of areas in the posterior hypothalamus do not. Ovulation can also be elicited by injections of sheep median-eminence extracts, and by preparations of sheep luteinizing hormone. Observations on the number of corpora lutea formed, and ripe Graafian follicles remaining after treatment, suggest that mating is the most potent stimulus for ovulation, followed by electrical stimulation and hormone injection.
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