Ferrocyanide was detected histologically in adult rat testes by the Prussian blue reaction from 3 min to 3 hr following intravenous injection. Ferrocyanide appeared in the lumen of the testicular blood vessels almost immediately following injection. After 10 min, ferrocyanide was detected in most seminiferous tubule walls and, 20 to 30 min later, Prussian blue deposits were localized in the lumen and epithelium of some seminiferous tubules. The results suggest that the continuity of the extratubular and intratubular ferrocyanide compartments is interrupted in part by tubular wall structures. Possible explanations for the intratubular diffusion of ferrocyanide are discussed.
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