AN ANTIGLOBULIN TEST FOR THE DETECTION OF SURFACE ANTIGENS OF MOUSE SPERMATOZOA

in Reproduction

Although a variety of methods have been used for measuring antibodies to spermatozoa, many have only been qualitative, for instance immunofluorescence. The most commonly used technique, sperm agglutination, would not measure non-precipitating antibodies. The more recently developed cytotoxic tests (Hamerlynck & Rümke, 1968) also detect a limited spectrum of antibody types. Binding tests which measure antibody absorbed to a target cell have rarely been used in studies of sperm antigens. Noyes (1969) used a direct binding test for human anti-sperm antibodies which involved labelling a patient's sera with131I. In the work described here, an isotopic antiglobulin technique (Harder & McKhann, 1968; Bomford, Breitner, Mitchison, Negroni & Raff, 1969; Sparks, Ting, Hammond & Herberman, 1969) has been adapted for use with mouse spermatozoa. This is an indirect or `sandwich' technique which utilizes125I-rabbit-anti-mouse-γ-globulin to detect mouse anti-mouse-spermatozoa antibodies bound to spermatozoa. The one radioactive reagent

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