The chemotactic activity for neutrophil leucocytes of the isolated spermatozoa and of the sperm-free seminal fluid was studied by a modification of Boyden's technique. Both spermatozoa and seminal fluid were found to attract neutrophils in the presence of serum or plasma, but not in their absence. The activity of seminal fluid is probably mediated in part by the complement system but it is unlikely that the classical haemolytic complement reaction is responsible for the activity of spermatozoa.
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