SOME METHODS FOR WASHING SPERMATOZOA FROM BULL, BOAR AND RAM: A COMPARISON USING BIOCHEMICAL AND OTHER CRITERIA

in Reproduction

Summary.

A study was made of three methods of washing bull, boar and ram spermatozoa, using as criteria contamination with free-lying cytoplasmic droplets, sperm motility and leakage of glucose-phosphate isomerase and hexokinase into the medium. The methods used were: washing in a Ringer-fructose-phosphate medium, washing in a hypoosmotic phosphate medium and washing with balanced media in the cold after slow cooling and storage overnight at 4° C in a milk diluent. Only by using the last method was it possible to remove adequately the freelying cytoplasmic droplets ; this method also caused least damage to the sperm suspensions.

The problems of washing spermatozoa with respect to cell damage and leakage of enzymes are discussed in the light of the present findings. The evidence suggests that glucose-phosphate isomerase and hexokinase leak from the cytoplasmic droplets as well as from the spermatozoa during washing. Hypo-osmotic media appear to cause an immediate and marked release of these enzymes from the droplets.

If the inline PDF is not rendering correctly, you can download the PDF file here.

 

     An official journal of

    Society for Reproduction and Fertility

 

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 99 14 0
PDF Downloads 267 88 2