Attempts were made to induce premature ovulation on the day of pro-oestrus in the 4-day cyclic rat by lh, fsh and/or prolactin administered a day earlier. Doses of 5 μg lh and 15 μg fsh, when given alone, induced partial ovulation in a few animals but the combination induced normal ovulation in 93% of the rats. Ovine prolactin given alone failed to induce ovulation and, when given in combination with lh, fsh or both, did not exert synergistic effects on ovulation. These data provide additional evidence that lh and fsh interact to induce ovulation in the normal oestrous cycle and that the ovulating hormone is composed of at least lh and fsh.
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