The following factors were found to influence the superovulatory response in immature mice and rats: (1) the dose of pregnant mares' serum (pms), (2) the dose of human chorionic gonadotropin (hcg), (3) the time interval between pms and hcg injections, and (4) the time interval between hcg and recovery of maximum number of ova. Dose-response curves for pms were bell-shaped in both species whereas increasing doses of hcg resulted in a plateau. Histological examination of the ovaries indicated that high doses of pms resulted in cystic follicles in the rat and preluteinized follicles in the mouse. Both conditions led to a failure to induce superovulation.
If the inline PDF is not rendering correctly, you can download the PDF file here.