Direct immunofluorescent staining of specimens of accessory sexual gland tissue and of coagulo-prostatic fluid, a normal secretory product of the coagulating and prostate glands of the male rabbit with fluorescein-labelled goat anti-rabbit IgG antiserum revealed localization of IgG within the folds of the submucosal stroma of acini of the coagulating and prostate glands. Binding of IgG to secretory granules within the lumina of these glands and presumably to these same granules seen in preparations of smears of coagulo-prostatic fluid was also seen. Similar immunofluorescent staining of the seminal vesicles disclosed localization of IgG to the stroma and faint intercellular or cytoplasmic fluorescence of glandular epithelial cells.
The possible implications of these findings relative to the development of autoantibodies to accessory sex gland tissues and fluids of the male rabbit and the means of acquisition of IgG by these tissues and fluids are discussed.
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