Damage to spermatogenesis in mice, rats, guinea-pigs, rabbits and rams after the intramuscular or subcutaneous administration of bull seminal vesicle fluid was reported by Matoušek (1966, 1969). Matoušek & Petrovská (1969) also observed an inhibitory effect of bull seminal vesicle fluid on the fertility of female mice and hens. The present report deals with the separation and purification of a substance that causes aspermatogenesis in mice.
Seminal vesicle fluid obtained from mature bulls at slaughter was diluted 2·5-fold with 2% acetic acid and mixed for 30 min at room temperature. The precipitate was separated from the supernatant fluid by centrifugation at 7000 g for 45 min. The aspermatogenic substance remained in the supernatant fluid. The bulk of the protein was then precipitated by 3 m-ammonium sulphate and separated from the supernatant by further
Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.