CULTURE OF EXPLANTED RAT EMBRYOS IN ROTATING TUBES

in Reproduction
Authors:
D. A. T. NEW
Search for other papers by D. A. T. NEW in
Current site
Google Scholar
PubMed
Close
,
P. T. COPPOLA
Search for other papers by P. T. COPPOLA in
Current site
Google Scholar
PubMed
Close
, and
S. TERRY
Search for other papers by S. TERRY in
Current site
Google Scholar
PubMed
Close
View More View Less
Free access

Various methods have been developed in recent years for growing post-implantation rat and mouse embryos in culture. Young embryos—up to early somite stages—grow well in static medium, e.g. simple watch-glass cultures (New, 1966; Steele, 1972). Older embryos require flowing medium, as in the culture systems of New (1967), Tamarin & Jones (1968), Robkin, Shepard & Tanimura (1972) and Cockroft (1973). These methods and some of their applications have been reviewed by New (1973).

Recently, a simpler technique for maintaining embryos in flowing medium has proved useful in this laboratory (New & Brent, 1972; New & Mizell, 1972). It consists of the culture of free-floating embryos in a constantly rotated tube which contains serum and an appropriate gas phase. We give here a short description of the method and a comparison of the results with those of the `circulator' method (New, 1967).

 

  • Collapse
  • Expand

     An official journal of

    Society for Reproduction and Fertility