VARIATION OF THE DNA CONTENT OF MORPHOLOGICALLY NORMAL AND ABNORMAL SPERMATOZOA IN MICE SUSCEPTIBLE TO IRREGULAR MEIOTIC SEGREGATION

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Authors:
R. STOLLA
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A. GROPP
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DOI:
https://doi.org/10.1530/jrf.0.0380335
Volume/Issue:
Volume 38: Issue 2
Page Range:
335–346
Article Type:
Research Article
Online Publication Date:
Jun 1974
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Summary.

The DNA content of morphologically normal spermatozoa from the laboratory mouse (Mus musculus), the tobacco mouse (M. poschiavinus) and M. musculus × M. poschiavinus F1 hybrids was determined by u.v. photometry. The DNA content was also determined in morphologically abnormal spermatozoa from F1 males.

In contrast to the parental strains, the F1 hybrids showed a high incidence of aneuploid secondary spermatocytes. This was due to irregular meiotic anaphase I segregation. The variation of the DNA content in morphologically normal spermatozoa was significantly higher in the F1 hybrids than in the laboratory mouse and the tobacco mouse. This was attributed to the presence of a considerable fraction of aneuploid spermatozoa with a hypo- or hypermodal variation of the haploid chromosomal complement.

The frequency of teratospermy was increased in F1 males. The morphologically abnormal spermatozoa showed a higher mean DNA content and a considerably larger standard deviation of the DNA content than that observed in morphologically normal spermatozoa. Spermatozoa carrying extreme deviations from the modal chromosome number seemed to be more frequent in this group than in the morphologically normal spermatozoa of the F1 males.

It is concluded that even though a majority of the aneuploid spermatogenic precursor cells may develop to morphologically normal (yet chromosomally unbalanced) spermatozoa, a minority may give rise to morphologically abnormal spermatozoa. There is evidence that neardiploid spermatozoa are due to a failure of the second meiotic division.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899