CHOLINESTERASE DISTRIBUTION DURING SPERMATID DIFFERENTIATION AND SPERMATOZOAL MATURATION IN THE WHITE MOUSE

in Reproduction
Authors:
JYOTSNA CHAKRABORTY
Search for other papers by JYOTSNA CHAKRABORTY in
Current site
Google Scholar
PubMed Close
and
L. NELSON
Search for other papers by L. NELSON in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1530/jrf.0.0380359
Volume/Issue:
Volume 38: Issue 2
Page Range:
359–367
Article Type:
Research Article
Online Publication Date:
Jun 1974
Free access

Sign up for journal news

Summary.

The reaction sites of acetyl and non-specific cholinesterases were located in the electron microscope in spermatogenic tissue and mature spermatozoa of the white mouse. A variety of substrates and inhibitors were used to distinguish between the two groups of enzymes. The shifting distribution of the cholinesterases during sperm cell development and maturation indicates that the origin and fate of the acetylcholine-cholinesterase system may be correlated with the initiation and control of sperm motility. During spermiogenesis, the cholinesterases first appeared in the plasma membrane of the early spermatid. With the gradual development of the spermatids, the reactivity increased; a large amount of reaction product appeared in the neck region, in the fibrillar components and in the cytoplasm of the maturing spermatozoa. As the spermatozoa were liberated into the lumen, much of the enzyme was discarded through the residual bodies. The smooth-surfaced endoplasmic reticulum of Sertoli cells, sub-surface cisternae and the filament bundle of the `mantle' always reacted strongly. In mature spermatozoa, the main reaction sites shifted to the fibrillar components. Some reactivity might also be noticed at the plasma membrane and mitochondrial surfaces. During maturation in the epididymis, the spermatozoa discarded an additional amount of enzyme along with the cytoplasmic droplet.

Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 69 14 1
PDF Downloads 53 16 1

 

  • Collapse
  • Expand
Print ISSN:
1470-1626
Online ISSN:
1741-7899