The epithelium lining a distal segment of the tubuli recti and neighbouring portions of the rete testis in the guinea-pig has been found to store large amounts of glycogen. The cell bodies are distended by a large central deposit of glycogen that displaces the nucleus far into the apex and reduces the cytoplasm to a thin peripheral layer adjacent to the cell membrane. Glycogen storage by these cells seems to have been overlooked until now because they break open and lose their content during routine histological preparation. Success in preserving and staining their glycogen is attributed to use of ferrocyanide-reduced osmium tetroxide after primary glutaraldehyde fixation.
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