Studies on the effects of pH on growth of preimplantation mammalian embryos have so far been limited to the mouse. Brinster (1965a) found that blastocysts developed in culture from two-cell mouse ova in media with pH values ranging from 5·87 to 7·78 with an apparent optimum of 6·82. He later found, however, that the optimum pH appeared to depend on the carbohydrate energy source present in the medium (Brinster, 1965b). These studies were carried out using bicarbonate-buffered media.
The work reported here extends the study of these problems to the rabbit, using a synthetic medium similar to that developed by Kane & Foote (1970) for culture of rabbit ova. One-cell rabbit ova were cultured in media of various bicarbonate concentrations. Two experiments were carried out.
Experiment 1 was a preliminary study in which bicarbonate concentrations were varied between 1·875
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