MATURATION AND FERTILIZATION IN VITRO OF GUINEA-PIG OVARIAN OOCYTES
in ReproductionSearch for other papers by R. YANAGIMACHI in
Current site
Google Scholar
PubMed
Mammalian ovarian oocytes can mature in vitro when liberated from Graafian follicles and placed in appropriate culture media. This was first demonstrated in the rabbit (Pincus & Enzmann, 1935, 1937; Chang, 1955a, b) and has been confirmed in a variety of animals (see Donahue, 1972; Biggers, 1973; Fowler & Edwards, 1973).
According to Jagiello (1969), 80 to 100% of guinea-pig oocytes isolated from ovaries of adult females in the middle (Days 5 to 8) of their oestrous cycle resume meiosis upon culture and may reach metaphase II by 14 hr of culture. Unfortunately, Jagiello gave no detailed information of the techniques which she used for isolation and culture of the oocytes and did not determine whether the oocytes matured in vitro were fertilizable. This paper describes a method of culturing
Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 198 | 80 | 11 |
| PDF Downloads | 118 | 44 | 4 |
Online ISSN: 1741-7899
Print ISSN: 1470-1626
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519