Utilization in vitro of [U-14C]glucose by spermatozoa of the domestic fowl, Gallus domesticus, in the presence of oviducal extracts from hens maintained under two environmental temperatures (19° and 31°C) was determined by radiorespirometry. It was found to be significantly greater in the presence of extracts obtained from the infundibulum than in any of the other extracts evaluated. Unlike those of other regions of the oviduct, extracts from the isthmus decreased glucose utilization by the spermatozoa. Extracts obtained from hens maintained at 31°C consistently had a greater stimulatory effect on glucose utilization by the spermatozoa (regardless of the source of the extract) as compared to extracts from hens maintained at 19°C.
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