ISOLATION AND LOCALIZATION OF AN IRON-BINDING RABBIT SEMINAL PLASMA ANTIGEN

M. G. O'RAND

doi:10.1530/jrf.0.0390349

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ISOLATION AND LOCALIZATION OF AN IRON-BINDING RABBIT SEMINAL PLASMA ANTIGEN

in Reproduction

Author:

M. G. O'RAND

M. G. O'RAND
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DOI:: https://doi.org/10.1530/jrf.0.0390349

Volume/Issue:: Volume 39: Issue 2

Page Range:: 349–357

Article Type:: Research Article

Online Publication Date:: Aug 1974

Free access

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Summary.

An iron-binding protein (IBP) is present in rabbit ejaculates. The IBP was isolated from rabbit ejaculates by ion-exchange and Sephadex G-100 chromatography. It had the electrophoretic mobility of a slow β-globulin and an approximate molecular weight of 105,000±10,000. Using specific antibodies, IBP was detected in seminal plasma from vasectomized males but not in epididymal sperm supernatants or extracts. Further, IBP could be localized on seminal particles by immunofluorescence microscopy and exchange agglutination. Rabbit IBP could not be detected on the surface of ejaculated or epididymal spermatozoa.

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