IMPORTANCE OF SPERM CAPACITATION FOR GAMETE CONTACT IN THE RABBIT
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The capacitation process has been suggested as a prerequisite for every stage of gamete interaction, including penetration of the granulosa cell investment (Austin, 1960), contact with the zona pellucida (Bedford, 1967; Hartmann & Gwatkin, 1971), penetration of the zona substance (Bedford, 1970) and, for the hamster at least, incorporation into the vitellus (Yanagimachi & Noda, 1970).
Morphological studies have demonstrated a characteristic fusion and vesiculation of the plasma membrane and outer acrosomal membrane of the spermatozoon during its passage through the granulosa cell mass (Barros, Bedford, Franklin & Austin, 1967). Capacitation is considered to be a prerequisite for this acrosome reaction and it may be inferred that membrane changes are related to capacitation as well (Bedford, 1969, 1970). It is uncertain,however, whether the
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