EFFECTS OF THE INCORPORATION OF TRITIATED ADENOSINE INTO MOUSE OVARIES ON OVULATION AND DEVELOPMENTAL CAPACITY OF OVA

in Reproduction
Author:
ROSEMARY BACHVAROVA
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The sensitivity of mammalian oocytes to irradiation has been recently summarized (Baker, 1971). This report describes the effects of β-radiation from [8-3H]adenosine incorporated into the ovary, on ovulation and on the developmental capacity of ovulated ova.

Female ICR mice which were 24 to 26 days old and had been reared in our colony were used in all experiments. Ovaries were exposed to [8-3H]adenosine by injecting 5 μl of a concentrated solution into the ovarian bursa. The precursor solution contained 12·5 to 50 μCi (Schwarz-Mann, 15 to 25 mCi/ μmol) in 5 μl sterile mammalian Ringer's solution buffered at pH 7 with 2 mm-phosphate. Using 25 μCi per ovary, the total uptake into the ovary reaches a peak of 7% of the injected precursor in 2 hr, and total

 

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