STEROID PRODUCTION BY THE ISOLATED RABBIT OVARIAN FOLLICLE

E. V. YOUNGLAI

doi:10.1530/jrf.0.0400095

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STEROID PRODUCTION BY THE ISOLATED RABBIT OVARIAN FOLLICLE

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E. V. YOUNGLAI

E. V. YOUNGLAI
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DOI:: https://doi.org/10.1530/jrf.0.0400095

Volume/Issue:: Volume 40: Issue 1

Page Range:: 95–103

Article Type:: Research Article

Online Publication Date:: Sep 1974

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Summary.

Graafian follicles from mature New Zealand white rabbits were incubated for 4 hr at 37°C with air as the gas phase. The medium was changed every 15 min for 1 hr. Medium containing ovine LH was then added for four consecutive medium changes. Samples of the medium were analysed for 17 β-hydroxyandrogens, oestrogens and progestins by radioimmunoassay procedures. When no LH was present, progestin and 17 β-hydroxyandrogen secretion declined to negligible levels within 2 hr. Addition of 10 μg ovine LH to the medium for 1 hr caused an increase in 17 β-hydroxyandrogen secretion which continued for the duration of the experiment. No significant change was observed in oestrogen secretion but there was a slight increase in progestin secretion. Addition of 5 μg and 1 μg LH for 15 min also caused an elevation of 17 β-hydroxyandrogen secretion within 1 hr whereas progestin secretion took longer to increase and no change in oestrogen secretion was observed. No effect was observed with LH concentrations of less than 500 pg/ml for 1 hr, but 1 and 10 ng LH/ml also caused an increase in 17 β-hydroxyandrogen secretion. Oestrogen secretion was also stimulated at these levels. Control incubations with interstitial tissue showed no oestrogen secretion, slight secretion of 17 β-hydroxyandrogens and greater secretion of progestin which was stimulated by exogenous LH.

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