FERTILIZATION AND DEVELOPMENT IN VIVO OF RABBIT OOCYTES CULTIVATED IN VITRO
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Pincus & Enzmann cultivated rabbit ovarian oocytes in vitro as early as 1935. In a study of the development of cultivated oocytes transferred to the oviducts of mated females, Chang (1955a) obtained 81% cleavage. In further experiments, Chang (1955b) evaluated embryonic development after the transplantation of cultivated oocytes and observed positive results with the oocytes isolated for cultivation from the ovaries 6 hr or more after injecting sheep pituitary extract. Thibault & Gérard (1970) fertilized cultivated oocytes in vitro and described the formation of anomalies in the development of the male pronuclei. The aim of the present study was to determine whether oocytes collected shortly after mating followed by an injection of HCG and then cultivated in vitro would be capable, after fertilization in the oviduct, of normal development in the
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