PREIMPLANTATION DEVELOPMENT IN VIVO AND IN VITRO IN BANK VOLES, CLETHRIONOMYS GLAREOLUS, TREATED WITH PMSG AND HCG

EWA T. MYSTKOWSKA

doi:10.1530/jrf.0.0420287

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PREIMPLANTATION DEVELOPMENT IN VIVO AND IN VITRO IN BANK VOLES, CLETHRIONOMYS GLAREOLUS, TREATED WITH PMSG AND HCG

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Author:

EWA T. MYSTKOWSKA

EWA T. MYSTKOWSKA
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DOI:: https://doi.org/10.1530/jrf.0.0420287

Volume/Issue:: Volume 42: Issue 2

Page Range:: 287–292

Article Type:: Research Article

Online Publication Date:: Feb 1975

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Summary.

Ovulation can be induced in the bank vole by PMSG and HCG and takes place 9½ to 11½ hr after administration of HCG. The number of eggs obtained varied from 1 to 30 (average 9·3). The highest ovulation rates were observed when PMSG and HCG were administered 41 to 43½ hr apart. The embryos attained the blastocyst stage 96 hr after HCG injection. Bank vole eggs can be cultured in chemically defined media beginning with the eight-cell stage; only 25% of fourcell eggs developed to blastocysts in vitro.

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