THE EFFECT OF OESTROGEN AND PROGESTERONE ON THE INCORPORATION OF TRITIATED THYMIDINE IN MOUSE UTERI IN VITRO

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Authors:
JAN CARTER
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ANNE McLAREN
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DOI:
https://doi.org/10.1530/jrf.0.0420439
Volume/Issue:
Volume 42: Issue 3
Page Range:
439–445
Article Type:
Research Article
Online Publication Date:
Mar 1975
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Summary.

The incorporation of tritiated thymidine into the uteri of immature mice in organ culture was assessed by autoradiography and liquid scintillation spectrometry. Oestrogenand progesterone, alone or combined, stimulated an increase in the incorporation of tritiated thymidine, indicating that DNA synthesis is occurring in vitro. Progesterone alone stimulated incorporation of label into stromal and epithelial cells, but the combination of progesterone and oestrogen stimulated the labelling of epithelial cells more than of stromal cells.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899