METABOLISM OF THE MOUSE PLACENTA
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Faculty of Veterinary Science, P.O Box 32, Khartoum North, Sudan
(Received 23rd July 1974)
Most of the studies of placental metabolism (Wang & Hellman, 1943; Page, 1948; Hellman, Harris & Andrews, 1950; Villee, 1953; MacKay, 1958; Friedman & Sachtleben, 1960; Baird & McDonald, 1964) have involved tissue slice preparations using Warburg's apparatus. In an isolated study by Villee & Hagerman (1953), homogenates of human placentae were prepared and their O2 utilization was tested using glucose, pyruvate, acetate, citrate, α-ketogluturate, succinate, malate and oxalacetate as substrates.
In the present study, an attempt was made to isolate mitochondria from mouse placentae at different stages of gestation and to study their metabolic activity by a polarographic method.
The experiment involved the use of 195 albino mice, 12 to 19 days pregnant. Animals at the same stage of pregnancy were killed by cervical dislocation, the placentae were removed and the mitochondria were prepared
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