THE ISOLATION AND CHARACTERIZATION OF LACTOFERRIN FROM SOW MILK AND BOAR SEMINAL PLASMA

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Authors:
T. K. ROBERTS
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and
J. C. BOURSNELL
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DOI:
https://doi.org/10.1530/jrf.0.0420579
Volume/Issue:
Volume 42: Issue 3
Page Range:
579–582
Article Type:
Research Article
Online Publication Date:
Mar 1975
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Summary.

Lactoferrin isolated from sow milk (about 0·6 mg/ml) was shown to be chromatographically homogeneous, an observation supported by electrophoresis and by reaction against monospecific anti-lactoferrin antiserum. Isoelectric focusing showed multiple forms of the protein (i.e.p., 9·3 to 10·0) converted by neuraminidase to one form (i.e.p., 9·65). Boar seminal plasma contains immunologically identical lactoferrin (0·1 to 0·5 mg/ml) which binds strongly to boar spermatozoa.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899