PLASMA LH AND PROGESTERONE LEVELS AFTER SINGLE OR MULTIPLE INJECTIONS OF SYNTHETIC LH-RH IN ANOESTROUS EWES AND COMPARISON WITH LEVELS DURING THE OESTROUS CYCLE

in Reproduction

Department of Physiology and Environmental Studies, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leicestershire

(Received 20th November 1974)

It has been shown that administration of a single intravenous injection of 150 or 300 μg synthetic LH-releasing hormone (LH-RH) to seasonally anoestrous Clun Forest ewes induced LH release in all animals and ovulation in the majority (Foster & Crighton, 1973, 1974). The induced LH peak was, however, much smaller than that observed at oestrus (Foster & Crighton, 1974). Luteal function, as assessed by peripheral plasma progesterone levels, was absent in the majority of treated animals (Crighton, Foster, Haresign, Haynes & Lamming, 1973; Haresign, Foster, Crighton, Haynes & Lamming, 1975), possibly because of an inadequate release of LH before ovulation. As an alternative means of administration to simulate more accurately the natural preovulatory LH peak, a single dose of 150 μg LH-RH was administered subcutaneously or intramuscularly instead of intravenously,

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