ISOELECTRIC FOCUSING OF BOAR SPERMATOZOA

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Authors:
H. D. M. MOORE
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and
K. G. HIBBITT
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DOI:
https://doi.org/10.1530/jrf.0.0440329
Volume/Issue:
Volume 44: Issue 2
Page Range:
329–332
Article Type:
Research Article
Online Publication Date:
Aug 1975
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Summary.

The isoelectric points of washed spermatozoa from intact boars and from boars after removal of the seminal vesicles were determined using isoelectric focusing on natural pH gradients. Normal boar spermatozoa focused at a higher pH than spermatozoa from boars without seminal vesicles. The isoelectric point of the latter was increased to a value approaching normal by preincubation in normal seminal plasma. This indicates that seminal plasma alters the membrane surface charge of boar spermatozoa on ejaculation.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899