PRESERVATION OF DIFFERENTIAL STAINING OF SPERMATOZOA BY FORMOL CITRATE

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Authors:
H. M. DOTT
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and
G. C. FOSTER
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DOI:
https://doi.org/10.1530/jrf.0.0450057
Volume/Issue:
Volume 45: Issue 1
Page Range:
57–60
Article Type:
Research Article
Online Publication Date:
Oct 1975
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Summary.

Semen from boar, bull, ram, rabbit, reindeer and stallion was diluted in formol citrate or formol saline and stained with eosin—nigrosin. The proportion of eosinophilic spermatozoa did not differ from that in fresh semen after storage for 48 hr in the formol diluent at temperatures ranging from 4°C to 40°C. Some samples were kept for periods up to 3 weeks with very little increase in the proportion of eosinophilic spermatozoa.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899