DEVELOPMENT OF RABBIT EMBRYOS IN VITRO AND IN VIVO FOLLOWING STORAGE OF THE TWO-CELL STAGE AT 10°C

in Reproduction
Authors:
GARY B. ANDERSON
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and
R. H. FOOTE
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DOI:
https://doi.org/10.1530/jrf.0.0450151
Volume/Issue:
Volume 45: Issue 1
Page Range:
151–153
Article Type:
Research Article
Online Publication Date:
Oct 1975
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Department of Animal Science, and Division of Biological Sciences, Cornell University, Ithaca, New York 14853, U.S.A. (Received 4th February 1975)

In 1947, Chang successfully stored young rabbit embryos for several days at 10°C. Recent interest in the practical application of embryo transfer techniques to livestock has resulted in renewed efforts to preserve mammalian embryos by refrigeration (Sreenan et al., 1970; Kardymowicz, 1972; Moore & Bilton, 1973; Anderson & Foote, 1975b), and by freezing (Wilmut & Rowson, 1973; Bank & Maurer, 1974; Whittingham & Whitten, 1974).

Anderson & Foote (1974, 1975a) reported that embryo metabolism is reduced while embryos are held at 10°C, but substrate utilization and normal DNA, RNA and protein synthesis is restored by rewarming to 37°C. The experiments reported in this paper were conducted to compare the relative development of embryos in vitro and in vivo following storage at low temperature.

Two-celled embryos from mature Dutch-Belted rabbits induced

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Print ISSN:
1470-1626
Online ISSN:
1741-7899