National Dairy Research Institute, Karnal, India (Received 25th February 1975)
Studies of buffalo semen have so far been directed towards eliciting its basic chemical composition and preserving the spermatozoa in vitro. Very little is known about the metabolism of buffalo spermatozoa except that they can oxidize fructose (Sengupta et al., 1963; Sinha et al., 1966). The observations made by us on the fructolysis and respiration of buffalo spermatozoa are presented in this paper because of their intrinsic comparative interest.
Semen was collected from Murrah buffalo bulls by using an artificial vagina. Pooled samples from one or two bulls whose spermatozoa showed good initial motility were used. Sperm counts were carried out in duplicate with a haemocytometer and the proportions of live spermatozoa were determined from smears stained with nigrosin-eosin. Motility was scored arbitrarily from 5 for vigorously motile to 0 for immotile spermatozoa; under anaerobic conditions, the spermatozoa were examined
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