Cyclic AMP in sheep ovarian follicles: site of production and response to gonadotrophins

in Reproduction
Authors:
T. J. Weiss
Search for other papers by T. J. Weiss in
Current site
Google Scholar
PubMed Close
,
R. F. Seamark
Search for other papers by R. F. Seamark in
Current site
Google Scholar
PubMed Close
,
J. E. A. McIntosh
Search for other papers by J. E. A. McIntosh in
Current site
Google Scholar
PubMed Close
, and
R. M. Moor
Search for other papers by R. M. Moor in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1530/jrf.0.0460347
Volume/Issue:
Volume 46: Issue 2
Page Range:
347–353
Article Type:
Research Article
Online Publication Date:
Mar 1976
Free access

Sign up for journal news

Summary.

This study was undertaken (i) to establish a relationship between cyclic AMP (cAMP) production and the degree of LH and FSH stimulation; (ii) to determine the effects of various gonadotrophins on follicular formation of cAMP; and (iii) to identify the precise intrafollicular site of cAMP formation.

The formation of cAMP increased rapidly in follicles exposed to LH. Maximum concentrations were reached after 90 min and were maintained for 180 min. Extracellular release of cAMP increased steadily throughout the 180-min experimental period. Tissue levels of cAMP increased proportionally and significantly when LH concentrations in the medium were increased from 0 to 200 mi.u. ml-1.

Tissue levels of cAMP were significantly increased by HCG, prostaglandin E-2 and noradrenaline, but not by prolactin, prostaglandin F-2α, serotonin or melatonin.

Cyclic AMP formation occurred predominantly in the thecal compartment; the membrana granulosa contributed less than 3 % of the total amount of cAMP formed after gonadotrophic stimulation. A significant amount of cAMP from the thecal cells was released into the extracellular compartment and appeared to pass into the granulosa cells.

Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 215 124 8
PDF Downloads 59 15 1

 

  • Collapse
  • Expand
Print ISSN:
1470-1626
Online ISSN:
1741-7899