The effects of prostaglandin E-1 and F-2α on maturation of mouse oocytes in vitro
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Summary.
The direct effects of prostaglandin E-1 (PGE-1) and F-2α (PGF-2α) on isolated mouse oocytes were examined in a microtube culture method. In oocytes exposed to PGE-1, meiotic resumption was suppressed at the anaphase I stage, presenting an irregular arrangement of the chromosomes along with the spindle fibres which were characterized by nondisjunction of the tetrads. PGF-2α was less potent in suppressing oocyte maturation than PGE-1. The critical time of the action of PGE-1 was between 7·5 and 12 hr of incubation.
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