Induction of ovulation in adult mice with a synthetic LH-RH (AY-24,031) and an analogue (AY-25,205)

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Authors:
U. K. Banik
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and
M. L. Givner
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DOI:
https://doi.org/10.1530/jrf.0.0470095
Volume/Issue:
Volume 47: Issue 1
Page Range:
95–96
Article Type:
Research Article
Online Publication Date:
May 1976
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Department of Endocrinology and Immunochemistry, Ayerst Research Laboratories, P.O. Box 6115, Montreal, Canada

Ovulation can be induced in mice and rats by various types and combinations of gonadotrophins, as well as by synthetic LH-RH following pretreatment with PMSG (see Stern & Schuetz, 1970; Gosden & Readhead, 1974). The present work was undertaken to establish whether LH-RH and an analogue could induce ovulation in dioestrous mice without pretreatment with PMSG.

Adult (30 ± 5 g) virgin Swiss albino mice were maintained in an air-conditioned room (24-25°C; 45-50% humidity) and exposed to equal periods of light and darkness. The decapeptides used were: (1) synthetic LH-RH (AY-24,031): H-Pyr-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, and (2) LH-RH analogue (AY-25,205): H-Pyr-His-Trp-Ser-Tyr-d-Ala-Leu-Arg-Pro-NH-CH2-CH3. The freeze-dried decapeptides were injected i.p. in 0·2 ml of 0·1% gelatin/0·9% saline between 15.00 and 15.30 hours on the day of dioestrus which was determined from examination of vaginal smears and recognition of the

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Print ISSN:
1470-1626
Online ISSN:
1741-7899