Fertilization of rat oocytes cultured in vitro from various stages of maturation

in Reproduction
Authors:
K. Niwa
Search for other papers by K. Niwa in
Current site
Google Scholar
PubMed Close
,
M. Miyake
Search for other papers by M. Miyake in
Current site
Google Scholar
PubMed Close
,
A. Iritani
Search for other papers by A. Iritani in
Current site
Google Scholar
PubMed Close
, and
Y. Nishikawa
Search for other papers by Y. Nishikawa in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1530/jrf.0.0470105
Volume/Issue:
Volume 47: Issue 1
Page Range:
105–106
Article Type:
Research Article
Online Publication Date:
May 1976
Free access

Sign up for journal news

Department of Animal Science, College of Agriculture, Kyoto University, Kyoto, Japan 606

In a previous study, it was shown that nuclear maturation from the germinal vesicle stage to division of the first polar body in rat oocytes could be completed in a defined medium, but that the proportion of oocytes penetrated and cleaved was low (Niwa & Chang, 1975), perhaps because of some fault in the maturation process. The present study was to determine the number of oocytes penetrated and cleaved in vitro when they were cultured at various stages during maturation in vivo.

White rats of the Wistar strain were used. Immature females (21-25 days old) were induced to superovulate by a s.c. injection of 10 i.u. PMSG (Serotropin: Teikoku-Zoki Co.) 48 hr before an i.p. injection of 10 i.u. HCG (Puberogen: Sankyo Co.). Oocytes were recovered at various times after the HCG injection according to the procedures described

Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 131 25 2
PDF Downloads 113 19 1

 

  • Collapse
  • Expand
Print ISSN:
1470-1626
Online ISSN:
1741-7899