Fluorometric assay of the protein content of mouse and rat embryos during preimplantation development

in Reproduction
Authors:
J. Schiffner
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and
H. Spielmann
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DOI:
https://doi.org/10.1530/jrf.0.0470145
Volume/Issue:
Volume 47: Issue 1
Page Range:
145–147
Article Type:
Research Article
Online Publication Date:
May 1976
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Abteilung Embryonal-Pharmakologie, Department of Pharmacology, Freie Universität Berlin, 1 Berlin 33, Thielallee 69/73, West Germany

Quantitative and qualitative aspects of protein synthesis during the preimplantation development of the mouse have been studied by several groups (Weitlauf & Greenwald, 1967; Brinster, 1971 ; Epstein & Smith, 1973, 1974). Because of methodological difficulties, however, little attention has been given to the problem of estimating the absolute amounts of protein found in mouse embryos at successive stages of development. The protein content of embryos from mice induced to superovulate has been determined for the first 5 days of development by a micro-Lowry technique (Brinster, 1967). The protein content decreased from 28 ng at the 1-celled stage to 20·5 ng at the morula stage, and there appeared to be a 15 % increase in total protein when the blastocyst was formed (23 ng). Using the same technique, Weitlauf (1973) reported a net increase of 52 % (23

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Print ISSN:
1470-1626
Online ISSN:
1741-7899