A histochemical study of succinate dehydrogenase in mouse oocytes and early embryos

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Authors:
E. Vivarelli
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,
G. Siracusa
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, and
F. Mangia
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DOI:
https://doi.org/10.1530/jrf.0.0470149
Volume/Issue:
Volume 47: Issue 1
Page Range:
149–NP
Article Type:
Research Article
Online Publication Date:
May 1976
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Institutes of Histology and General Embryology, University of Rome, and School of Medicine of L'Aquila, Italy

Ishida & Chang (1965) found that hamster and rabbit embryos show a gradual increase of the activity of the mitochondrial enzyme succinate dehydrogenase (SDH: E.C. 1.3.99.1) between the 1- and 8-cell stages, followed by a steeper rise at blastulation. Baranska, Dorywalski & Kujawa (1973) reported that SDH activity increases from the 1- to 4-celled stage and decreases in the morula and blastocyst in the mouse. In both these studies SDH was demonstrated by the Nachlas method (Nachlas, Tsou, Souza, Cheng & Seligman, 1957), a histochemical reaction based on the reduction of nitro blue tetrazolium (NBT), in which no intermediate electron acceptor between SDH and the tetrazolium dye is used. However, Horwitz, Benitez & Bray (1967) have shown that SDH cannot transfer electrons directly to NBT, but only by way of coenzyme Q or another

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Print ISSN:
1470-1626
Online ISSN:
1741-7899