Summary. The concentration of oestradiol was measured by radioimmunoassay in ovarian venous plasma collected from ewes of three breeds (Finnish Landrace, Scottish Blackface and Tasmanian Merino) on Day 9 of the oestrous cycle and in jugular venous plasma collected daily around oestrus in two of these breeds. The mean ± s.e.m. concentration in the ovarian venous plasma of the Merino (44·1 ± 7·6 pg/ml) was lower than that in Blackface (72·2 ± 10·2 pg/ml) and Finn ewes (66·8 ± 10·2 pg/ml) The overall fitted mean concentration in peripheral venous plasma was 1·7 pg oestradiol-17β/ml, with no difference between the Finn and Blackface ewes, in which the highest preovulatory values were 3·9 0·5 and 0·5 pg/ml respectively.
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