The effect of zinc ions on fertilization of mouse ova in vitro
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Summary. The fertilization rate of mouse gametes in a Krebs–Ringer bicarbonate medium was 60%. The rate fell to 0% when 250 μm-ZnCl2 was added, but MgCl2, CaCl2, MnCl2, FeCl2 and CuCl2 (each at 250 μm) had no effect. The inhibitory effect of Zn2+ was not evident if the spermatozoa or eggs were preincubated with Zn2+ or if previously capacitated spermatozoa were used. The Zn2+ is thought to act by interference with capacitation.
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