Estimation of progesterone in buffalo milk and its application to pregnancy diagnosis

in Reproduction
Authors: A. Singh and R. Puthiyandy
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Summary. Milk samples were collected on Days 20, 24, 28 and 40 after insemination. The percentage of fat and the progesterone concentration in the milk were correlated (P < 0·01). Progesterone concentration in the milk of pregnant buffaloes (24·83 ± 3·85 ng/ml) was significantly higher than that in non-pregnant animals (2·89 ± 1·25 ng/ml) on Day 20 and the difference between the two increased with time after insemination. The detection of non-pregnant animals was 100% successful at all times but the diagnosis was correct for 66, 68, 81 and 83% of animals tested on Days 20, 24, 28 and 40 respectively and predicted as pregnant.

 

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