Effects of putative protease inhibitors on the acrosome reaction of sea urchin spermatozoa
in ReproductionSearch for other papers by R. V. Hyne in
Current site
Google Scholar
PubMed
Search for other papers by D. L. Garbers in
Current site
Google Scholar
PubMed
Summary. The acrosome reaction was induced by jelly coat factors, nigericin, or elevated pH. When spermatozoa were preincubated for 5 min in sea water maintained at pH 7·9 in the presence of 1 mm-phenylmethylsulphonyl-flouride (PMSF), 1 mm-benzamidine, 0·1 mm-1-chloro-3-tosyl-amido-7-amino-2-heptanone (TLCK), 5 mm-diisopropylphosphofluoridate (DFP) or 5 mm-DFP that was previously hydrolysed, only DFP or its hydrolysis product(s) prevented formation of the acrosomal filament induced by jelly coat factors. When incubation with inhibitors was extended to 2 h only TLCK and its hydrolysis products inhibited the jelly-induced acrosome reaction. Only DFP significantly inhibited the acrosome reaction induced by elevated pH (9·0). Nigericin induced acrosome reactions in the presence of DFP or TLCK. These findings do not support the concept of an active role for acrosin in development of an acrosome reaction.
Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 120 | 29 | 0 |
| PDF Downloads | 73 | 29 | 3 |
Online ISSN: 1741-7899
Print ISSN: 1470-1626
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519