In-vitro fertilization in the mouse and the relevance of different sperm/egg concentrations and volumes
in ReproductionSearch for other papers by A. K. S. Siddiquey in
Current site
Google Scholar
PubMed
Search for other papers by J. Cohen in
Current site
Google Scholar
PubMed
Summary. The sperm:egg ratio and sperm concentration were varied separately by using different volumes (1–100 μl) and egg numbers (1–15). The % fertilization was determined by sperm concentration in large volumes (10–100 μl). In small volumes (1 or 5 μl) the number of spermatozoa/egg determined penetration rate; the effective epididymal sperm number/fertilization was 250–350. Spermatozoa obtained from the oviducts (i.e. naturally selected) gave good fertilization responses and low concentrations of epididymal spermatozoa were also more effective when oviducal fluid and cumulus cells were included in the inseminating volume. We conclude that sperm/egg collision rate is the most important factor determining % fertilization, although heterogeneity of epididymal spermatozoa limits fertilization rate at low sperm:egg ratios.
Reproduction is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 159 | 35 | 10 |
| PDF Downloads | 205 | 45 | 9 |
Online ISSN: 1741-7899
Print ISSN: 1470-1626
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519