Relationship between semen characteristics and fertility in electroejaculated mice

in Reproduction

Summary. Ejaculates were obtained from C57BL mice by applying two successive series of electrical stimuli which were delivered via a bipolar rectal probe. The ejaculates thus collected contained fertile spermatozoa as indicated by results from in-vitro fertilization. Once separated from the seminal plasma, ejaculated spermatozoa possessed the same in-vitro fertilization rate as epididymal sperm. Ejaculates were analysed for coagulum weight, ejaculate volume, sperm count, sperm motility, acid phosphatase content and fructose content. Significant differences were present between several of these values for fertile and infertile mice, and values were therefore empirically assigned to represent minimal amounts for 'normal' fertility (1·5 μl ejaculate volume; 10·2 mg coagulum weight; 2·5 × 106 spermatozoa/ml; 2·3 × 103 motile spermatozoa/ejaculate). One half of the fertile animals had no deficiencies in any of the characteristics measured, whereas 97% of the infertile animals had at least one deficiency. No fertile male had more than 2 deficiencies. These data show that the characteristics of mouse semen obtained by the present method of electroejaculation are related to the fertility status of the animal.

If the inline PDF is not rendering correctly, you can download the PDF file here.


    Society for Reproduction and Fertility

Related Articles

Article Information


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 262 208 5
PDF Downloads 568 522 11


Cited By


Google Scholar