Mechanism of inhibition by gossypol of glycolysis and motility of monkey spermatozoa in vitro
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Summary. Low levels of gossypol inhibited motility and anaerobic glycolysis of ejaculated rhesus monkey spermatozoa. Inhibition (50%) of both occurred at a gossypol:sperm ratio of 8 nmol/108 spermatozoa, and complete inhibition of both occurred at a ratio of 75 nmol/108 spermatozoa. Determination and comparison of the levels of glycolytic intermediates in intact spermatozoa, incubated with and without gossypol, indicated that the only site of glycolytic inhibition was lactate dehydrogenase-X (EC 1.1.1.27). At gossypol:sperm ratios above 6 nmol/108 spermatozoa, gossypol also decreased the concentrations of the adenine nucleotides, ATP, ADP and AMP, and this is most probably the basis for its toxic effect on spermatozoa.
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