Localization of epididymal secretory proteins on rat spermatozoa

in Reproduction
Authors:
D. E. Brooks
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K. Tiver
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DOI:
https://doi.org/10.1530/jrf.0.0690651
Volume/Issue:
Volume 69: Issue 2
Page Range:
651–657
Article Type:
Research Article
Online Publication Date:
Nov 1983
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Summary. Spermatozoa from the testis and cauda epididymidis of the rat were surface labelled with radioactive iodide. Detergent extracts of radioiodinated spermatozoa immunoprecipitated with antisera against specific epididymal proteins, followed by polyacrylamide gel electrophoresis, revealed two proteins (D and E of Mr 27 000 and 28 000, respectively) which became associated with spermatozoa during epididymal transit. These proteins were observed by immunofluorescence microscopy to be located over a restricted area of the head surface. Proteins with similar molecular weight were labelled on spermatozoa from the cauda epididymidis, but not from the testis, by reaction with sodium boro[3H]hydride in the presence of galactose oxidase. However, failure to immunoprecipitate with antibodies to Proteins D and E and non-coincident migration on two-dimensional gel electrophoresis established the non-identity of these proteins. Compared with Proteins D and E, two other major epididymal secretory proteins (Proteins B and C of Mr 16 000) associated with spermatozoa to a relatively minor extent during epididymal transit.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899