Study using in-vivo binding of 125I-labelled hCG, light and electron microscopy of the repopulation of rat Leydig cells after destruction due to administration of ethylene-1,2-dimethanesulphonate

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Authors:
N. C. Jackson
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,
H. Jackson
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J. H. Shanks
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J. S. Dixon
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, and
R. G. Lendon
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DOI:
https://doi.org/10.1530/jrf.0.0760001
Volume/Issue:
Volume 76: Issue 1
Page Range:
1–10
Article Type:
Research Article
Online Publication Date:
Jan 1986
Free access

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Summary. Gonadotrophin binding to rat Leydig cells after a single administration of ethylene dimethanesulphonate (EDS) (75mg/kg i.p.) was followed by using intratesticular microdoses of 125I-labelled hCG, whilst corresponding morphological changes in the testicular interstitium were studied with light and electron microscopy. No discernible effect on 125I-labelled hCG binding compared with controls was observed until 24 h after treatment. Between 24 and 32 h a sharp decline in binding occurred which was correlated with extensive Leydig cell destruction. By 48 h the 125I-labelled hCG binding was negligible and no morphologically recognizable Leydig cells were found at this time. The specific binding remained low until 21 days after treatment and then a marked increase occurred to give nearly normal levels by 49 days. This was consistent with a generalized repopulation of the interstitium with Leydig cells, seemingly the result of differentiation of fibroblast-like precursor cells.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899